Mammalian synthetic circuits with RNA binding proteins delivered by RNA
نویسندگان
چکیده
Expression kinetics of BHK21 cells transfected with two VEE replicons. (a) Replicons encoding constitutive EGFP and mKate were co-electroporated into BHK21 cells, and EGFP and mKate fluorescence levels were measured by flow cytometry at 3h, 6h, 12h, day 1, day 2, day 3, day 4, day 5, day 6, and day 7. (b) The percentage of EGFP/mKate double positive, EGFP single positive, mKate single positive, and double negative cells at the time points in (a) are plotted.
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عنوان ژورنال:
دوره 33 شماره
صفحات -
تاریخ انتشار 2015